Intracytoplasmic single Sperm Microinjection(ICSI), commonly known as second-generation IVF, uses micromanipulation techniques to inject sperm into the cytoplasm of the egg cell, fertilize the egg, culture it to early embryos in vitro, and then place it back into the mother's womb for development and implantation.

Applies to:

1. Severe male infertility (must be confirmed by more than three tests) include oligozoospermia, asthenospermia, teratospermia, and some azoospermia.

2. Fertilization failure or extremely low fertilization rate during treatment.

3. ICSI assisted fertilization is required after cryopreservation or immature eggs are matured in vitro.

4. Patients with linked diseases, chromosomal balance shift,or globin production-associated anemia require ICSI in pre-implantation diagnostic techniques.

5. Repeated IVF losers.